Standard VDPV screening assays may yield false-negative results for certain vaccine-derived poliovirus strains, highlighting the need for complete sequencing in suspicious cases.
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Caution with standard VDPV assays in atypical cases; leaves open whether sequencing should supplement surveillance.
(GPLN) and the World Health Organization’s Polio Eradi-cation Initiative (GPEI) accord high priority to detecting all vaccine-derived polioviruses (VDPVs) because they are neurovirulent and have the potential to cause outbreaks of poliomyelitis and establish poliovirus circulation. In pa-tients with immunodeficiency diseases, persistent infec-tions may become established with live oral poliovirus vaccine (OPV) and develop into VDPVs (1). Laboratories of the GPLN use standard procedures for virus isolation, identification, and intratypic differentiation (2). The real-time reverse transcription PCR (rRT-PCR) VDPV screen-ing assay became available to the GPLN in 2009. Polio-virus isolates that do not become amplified in the VDPV assay are subjected to complete sequencing of viral protein (VP) 1. VDPVs are isolates of Sabin OPV origin that have
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Sharma et al. (2015) studied this question.
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