Summary. Capacitation of mouse spermatozoa in vitro is brought about by epididymal secretions released into the medium at the time of sperm collection. Inhibition by glucaro(1→4)lactone indicates that an essential component of these secretions is β-glucuronidase. In the absence of the secretions, capacitation can be induced by components of the cumulus oophorus.
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Gwatkin et al. (1974) studied this question.
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