Ejaculates, collected over a 35-month period from 3 Polish Landrace boars from 8 months of age, were diluted (30 x 10 6 sperm/cm 3 ) with a standard extender, Kortowo 3 (K3) supplemented with lipoprotein fractions extracted from hen (K3-LPFh) or ostrich egg yolk (K3-LPFo). Thereafter, the diluted sperm samples were stored for 4 days (DO to D4) at 5 and 16C, respectively. Irrespective of the extender composition and storage temperatures, sperm motility was gradually reduced during prolonged storage. The decline in sperm motility was more accentuated in K3 extender compared with LPF-based extenders. Seasonal influence was more apparent in K3-LPFo extender during storage at 5C.
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Fraser et al. (2002) studied this question.