In vivo time‐resolved Raman imaging of a spontaneous death process of a single budding yeast cell

In vivo confocal Raman microspectroscopy has been used to study a spontaneous death process of a single budding yeast ( S. cerevisiae ) cell. The observed time‐resolved Raman images indicate that, concomitantly with the sudden appearance of a dancing body in a vacuole, the mitochondrial metabolic activity of the cell is markedly lowered and that the subsequent disappearance of the vacuole is followed by an inevitable cell death within a few hours.