Rana catesbeiana tadpoles immunized with the main hemoglobin component of the adult frog of the same species produced precipitating and agglutinating antibodies against the immunogen. After natural metamorphosis, the immunized froglets have as their only major hemoglobin a protein immunologically and electrophoretically different from the major hemoglobin of control froglets.
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Maniatis et al. (1969) studied this question.
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