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The isolation, synthesis and development of practical methods of assay have given a great stimulus to the study of vitamin C.¹More papers have appeared on methods of assay and distribution for vitamin C than for all the other vitamins combined. Therefore, no attempt is made here to present a systematic review of the literature. This has been done amply in the monograph by Sherman and Smith,²the yearly volumes of theAnnual Review of Biochemistry,³the bulletin of the United States Department of Agriculture on "Vitamin Content of Foods"⁴and numerous reviews.⁵The facts and comments presented in this review may serve to give a general idea of the present state of the subject. BIO-ASSAY Chemical methods have rapidly replaced vitamin C determinations by bio-assay for many types of investigations. However, the more specific animal tests continue to be necessary in order to avoid
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Otto A. Bessey (1938) studied this question.
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