Myosin subfragment binding for the localization of actin-like microfilaments in cultured cells. A light and electron microscope study.
Population
Cultured cells including rabbit skeletal muscle actin, rat embryo cells, and rat kangaroo cells
Comparison
Fluorescein-labeled heavy meromyosin… vs Pyrophosphate and competition with excess…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports actin visualization in cultured cells; leaves open functional validation in mammalian cardiac models.
Structured PICO
P
Population
Cultured cells including rabbit skeletal muscle actin, rat embryo cells, and rat kangaroo cells
I
Intervention
Fluorescein-labeled heavy meromyosin subfragment-1 (F-S-1) staining
C
Comparator
Pyrophosphate and competition with excess unlabeled S-1
O
Outcome
Localization and distribution of actin-containing structures (microfilaments) in interphase and mitotic cellssurrogate
Fluorescein-labeled heavy meromyosin subfragment-1 is an effective tool for localizing actin microfilaments in cultured cells, providing evidence for actin's structural and functional roles in the mitotic apparatus.
Cite This Study
Schloss et al. (1977) studied this question.