Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes

A synthetic medium has been developed in which Streptococcus faecalis var. zymogenes X-14 elaborates a lysin. The medium consists of 18 amino acids, lactose, six vitamins, adenine, guanine, uracil, and six salts. Glucose, and K(2)HPO(4) in excess of 0.5% (wt/vol), were inhibitory to lysin production; increasing concentrations of l-arginine-hydrochloride gave increasing yields of lytic activity.