Differences in the Behavior of Attached and Floating Cells Subjected to Low Intensity Ultrasound

Through mechanical considerations, we will try to determine whether cancer cells die because of resonance or energy present in solution. Attached and floating cells were stressed at fixed frequencies in a range between 400 kHz and 620 kHz at 10 PRF (Pulse Repetition Frequency). In the floating cells the power and the mortality show similar variations with respect to frequency and this allows to assume a direct relationship between power and mortality. The same experiment was replicated on attached cells by exposing the cultures at US of fixed frequency (between 400 and 620 kHz, with 10 Hz Pulses Repetition Frequency) either keeping constant power output or voltage. Cell mortality was found to be more sensitive to the frequency.