In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants

A protocol is established for regeneration of the economically important cut flower plant, Gypsophila paniculata L., using shoot tips explants. Multiple shoots were obtained on Murashige and Skoog medium fortified with 0.5 mg dm-3 each of α-naphthaleneacetic acid and 6-benzyladenine. Addition of 10 g dm-3 agar promoted shoot proliferation and reduced the degree of shoot vitrification. Transfer to 3 mg dm-3 indole-3-butyric acid containing medium produced optimum root initiation and development. The produced plants as well as intact plants were subjected to the random amplified polymorphic DNA (RAPD) analysis. Using 9 primers, the total number of amplification products generated by polymerase chain reaction was 142 bands (15.7 bands per primer), of which 7.74 % showed polymorphism. The analysis of bands recorded, showed 92.25 % similarity. The results indicated that very low variation at the DNA level occurred during in vitro culture of Gypsophila.