The rapid development of CRISPR/CRISPR-associated enzyme (Cas) technology has enabled truly customised treatment of human genetic disorders, paving the way for recent developments in the field of gene therapy. Because CRISPR/Cas can accurately target and edit individual genes within a genome, it has established itself as a formidable tool for genetic manipulation. Based on this natural process, the CRIPSR technology enables editing of target-specific DNA sequences in any organism's genome with just three molecules: the target DNA; an RNA guide that guides the complex to the target; and a nuclease, specifically caspase 9, which cleaves double-stranded DNA. CRISPR/Cas9 is a simple two-component system for effective targeted gene editing. CRISPR/Cas9 is becoming into a potent tool for high throughput target gene screening in cancer treatment. Genome editing using CRISPR/Cas9 technologies has becoming more and more popular.
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Shrikant P. Pawar (2025) studied this question.
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