Multifunctional glucose-6-phosphatase studied in permeable isolated hepatocytes.

Glucose-6-phosphatase in Isolated Hepatocytesreticulum, where approximately 80% of such activity resides (Gunderson and Nordlie, 1975).Results of these studies are presented here.' Experiments as depicted in Table I were done both using Krebs-Ringer buffer and equivalent, isotonic concentration (0.22 M ) of Hepes buffer.Patterns of results obtained were in good agreement.In subsequent studies, Hepes buffer was employed routinely, since Krebs-Ringer buffer contains anions competitively inhibitory to g hcose-6-phosphatase (see "Experimental Procedures") and since earlier studies with microsomal and nuclear preparations had been carried out with Hepes.The abbreviations used are: Hepes, 4-(2-hydroxymethyl)-1-piperazineethanesulfonic acid; DOC, deoxycholate.