Differential‐Frequency Saturation Transfer Difference NMR Spectroscopy Allows the Detection of Different Ligand–DNA Binding Modes

Vive la différence! The binding of small molecules of pharmaceutical interest to DNA can be screened by differential-frequency saturation transfer difference spectroscopy (DF-STD). The technique allows the probing of DNA recognition phenomena, such as those of base-pair intercalators, minor groove binders, and external backbone binders (see picture).