Unique Genetic and Histological Signatures of Mouse Pericardial Adipose Tissue

Figures

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  Figure 1. The location of epicardial adipose tissue (EAT), pericardial adipose tissue (PAT) and perivascular adipose tissue (PVAT) associated with the heart in rodents (A) and diagrammatically in humans (B). EAT is absent in the heart of rodents but present in the heart of human.

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  Figure 2. FABP4 (A), PPARγ (B), C/EBPα (C), IL6 (D), TNFα (E), Leptin (F), AdipoQ (G), UCP1 (H), ADRβ3 (I), DIO2 (J), PGC1α (K), COX7A1 (L) and COX8B (M) fold change in mRNA expression in gWAT, iWAT, iBAT and PAT of chow (C)-fed 30-week-old mice. All data are relative to the housekeeping gene PPIA and normalised to C-gWAT. All data represent mean + s.e.m. Data were analysed by one-way ANOVA and Tukey’s multiple comparison test. N = 8–12. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

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  Figure 3. Leptin (A) and AdipoQ (B) fold change in mRNA expression in gWAT, iWAT, iBAT and PAT of chow (C)- or high-fat (HF)-fed 30-week-old male mice. All data are relative to the housekeeping gene PPIA and normalised to their respective control (C) within each depot. All data represent mean + s.e.m. Data were analysed by an unpaired t-test between C and HF of each depot. N = 6–12. * p < 0.05 and ** p < 0.01.

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  Figure 4. IL6 (A) and TNFα (B) fold change in mRNA expression in gWAT, iWAT, iBAT and PAT of chow (C)- or high-fat (HF)-fed 30-week-old male mice. All data are relative to the housekeeping gene PPIA and normalised to their respective control (C) within each depot. All data represent mean + s.e.m. Data were analysed by an unpaired t-test between C and HF of each depot. N = 6–12. * p < 0.05 and **** p < 0.0001.

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  Figure 5. UCP1 (A), ADRβ3 (B) and DIO2 (C) fold change in mRNA expression in gWAT, iWAT, iBAT and PAT of chow (C)- or high-fat (HF)-fed 30-week-old male mice. All data are relative to the housekeeping gene PPIA and normalised to their respective control (C) within each depot. All data represent mean + s.e.m. Data were analysed by an unpaired t-test between C and HF of each depot. N = 6–12. * p < 0.05, ** p < 0.01 and *** p < 0.001.

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  Figure 6. IL6 (A), TNFα (B), Leptin (C) and UCP1 (D) fold change in mRNA expression in gWAT, iWAT, iBAT and PAT of high-fat (HF)-fed 30-week-old male mice. All data are relative to the housekeeping gene PPIA and normalised to HF-gWAT. All data represent mean + s.e.m. Data were analysed by one-way ANOVA and Tukey’s multiple comparison test. N = 6–8. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

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  Figure 7. Percentage change in adipocyte volume (a.u.) of gWAT, iWAT and PAT from 30-week-old chow (C)-fed mice. Data normalised to C-gWAT. Data were analysed by one-way ANOVA and Tukey’s multiple comparison test. N = 8–11 (A). Percentage change in adipocyte volume of chow (C)- or high-fat (HF)-fed 30-week-old mice from gWAT, iWAT and PAT. Data normalised to their respective control (C) within each depot. Data were analysed by an unpaired t-test between C and HF of each depot. N = 6–11 (B). Representative H&E stained images of chow (C)- or high-fat (HF)-fed 30-week-old mice from gWAT, iWAT and PAT. All scale bars 100µM. N = 6–11 (C). Distribution of adipocyte volume of chow (C)- or high-fat (HF)-fed 30-week-old mice from gWAT, iWAT and PAT. Data were analysed by one-way ANOVA and Tukey’s multiple comparison test between gWAT, iWAT and PAT of each cell volume (a.u.) bin. N = 6-11 (D). All data represent mean + s.e.m (A,B,D). * p < 0.05, ** p < 0.01 and *** p < 0.001 (A,B,D).