What question did this study set out to answer?

To characterize lysophosphatidic acid-activated chloride channel currents in human lung fibroblasts and evaluate their role in fibroblast-to-myofibroblast differentiation.

January 29, 2005AJP Lung Cellular and Molecular Physiology

Chloride channel activity in human lung fibroblasts and myofibroblasts

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Why the study?

Does chloride channel activity influence the differentiation of human lung fibroblasts to myofibroblasts?

Population

Cultured human lung fibroblasts (HLF) and myofibroblasts

Comparison

Activation of I using lysophosphatidic acid or… vs Control fibroblasts or untreated cells

Design

Preclinical

Authors

ZYZhaohong YinNanfang Hospital MWMitchell A. WatskyAugusta University

Discussion

Member takes

Implication

Chloride channel activity may influence fibroblast differentiation; leaves open therapeutic targeting in pulmonary fibrosis.

Key Points

To characterize lysophosphatidic acid-activated chloride channel currents in human lung fibroblasts and evaluate their role in fibroblast-to-myofibroblast differentiation.
Cultured human lung fibroblasts under control conditions or stimulated with transforming growth factor-beta to induce myofibroblast differentiation.
Recorded chloride currents activated by lysophosphatidic acid or sphingosine-1-phosphate using the amphotericin perforated-patch technique.
Evaluated receptor expression by RT-PCR and quantified alpha-smooth muscle actin levels using Western blotting and immunohistochemistry in the presence of chloride channel blockers.
Transforming growth factor-beta-induced myofibroblasts exhibited significantly higher alpha-smooth muscle actin levels and chloride current density than undifferentiated fibroblasts.
Chloride current activation was suppressed by channel blockers DIDS and NPPB, as well as the specific lysophosphatidic acid receptor antagonist dioctyl-glycerol pyrophosphate.
Treatment with DIDS and NPPB caused a significant, dose-dependent reduction in alpha-smooth muscle actin levels in growth factor-stimulated cells.

Structured PICO

Does chloride channel activity influence the differentiation of human lung fibroblasts to myofibroblasts?

Population

Cultured human lung fibroblasts (HLF) and myofibroblasts

Intervention

Activation of I(Cl-LPA) using lysophosphatidic acid (LPA) or sphingosine-1-phosphate, and blockade using DIDS, NPPB, and dioctyl-glycerol pyrophosphate

Comparator

Control fibroblasts (not cultured in TGF-beta) or untreated cells

Outcome

I(Cl-LPA) current density and alpha-smooth muscle actin (SMA) levels (fibroblast-to-myofibroblast differentiation)surrogate

Receptor-mediated activation of chloride channels by LPA and sphingosine-1-phosphate plays a critical role in the differentiation of human lung fibroblasts to myofibroblasts.

Cite This Study

Yin et al. (2005) studied this question.

synapsesocial.com/papers/6a82b24d1d8eddfbf50f6ef3 https://doi.org/10.1152/ajplung.00344.2004

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Chloride channel activity in human lung fibroblasts and myofibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Chloride channel activity in human lung fibroblasts and myofibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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