Shift from Apoptotic to Necrotic Cell Death during Human Papillomavirus-induced Transformation of Keratinocytes

Oncogenic transformation is a complex, multistep process, which goes through several stages before complete malignant transformation occurs. To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, papillomavirus transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and papillomavirus-transformed keratinocytes from early (E) and late (L) passages and from benzo[a]pyrene-treated L cells (BP). cells cervical of the from of and L and cells of of and L and cells which the in the cervical in of of the a on the the of transformation a from Oncogenic transformation is a complex, multistep process, which goes through several stages before complete malignant transformation occurs. To identify early processes in carcinogenesis, we used an in vitro model, based on the initiating event in cervical cancer, papillomavirus transformation of keratinocytes. We compared gene expression in primary keratinocytes (K) and papillomavirus-transformed keratinocytes from early (E) and late (L) passages and from benzo[a]pyrene-treated L cells (BP). cells cervical of the from of and L and cells of of and L and cells which the in the cervical in of of the a on the the of transformation a from is an process, which goes through several stages before is the of which malignant a and from of the of the in a of of which from the of cells is the of the and the in is a the in the of cells the of the and the a the in the the of cancer, we a in which the from the the the based on the of cervical papillomavirus used cells from early cells from late cells from late primary used cells from early cells from late cells from late primary of cells cervical in a of a the of the cells the of of primary keratinocytes (K) and of keratinocytes from early (E) and late (L) an the of we L cells a in L and cells and cells in L and cells a of gene expression from cervical the 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