Formyltetrahydrofolate Decarbonylase Synthesizes the Active Site CO Ligand of O ₂ -Tolerant [NiFe] Hydrogenase

[NiFe] hydrogenases catalyze the reversible oxidation of molecular hydrogen into two protons and two electrons. A key organometallic chemistry feature of the NiFe active site is that the iron atom is co-coordinated by two cyanides (CN – ) and one carbon monoxide (CO) ligand. Biosynthesis of the NiFe(CN) 2 (CO) cofactor requires the activity of at least six maturation proteins, designated HypA–F. An additional maturase, HypX, is required for CO ligand synthesis under aerobic conditions, and preliminary in vivo data indicated that HypX releases CO using N 10 -formyltetrahydrofolate ( N 10 -formyl-THF) as the substrate. HypX has a bipartite structure composed of an N-terminal module similar to N 10 -formyl-THF transferases and a C-terminal module homologous to enoyl-CoA hydratases/isomerases. This composition suggested that CO production takes place in two consecutive reactions. Here, we present in vitro evidence that purified HypX first transfers the formyl group of N 10 -formyl-THF to produce formyl-coenzyme A (formyl-CoA) as a central reaction intermediate. In a second step, formyl-CoA is decarbonylated, resulting in free CoA and carbon monoxide. Purified HypX proved to be metal-free, which makes it a unique catalyst among the group of CO-releasing enzymes.