Identification of regulatory regions and SNPs in the porcine GPIHBP1 promoter provides a resource for using pigs as a model for human cardiovascular disease research.
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Provides foundation for porcine CVD modeling; leaves open translation of GPIHBP1 SNPs to human outcomes.
GPIHBP1 is expressed restrictively in capillary endothelial cells, but the transcriptional regulation mechanisms of GPIHBP1 in capillaries are still unknown. Here, we analyzed the promoter activities of porcine GPIHBP1 using dual luciferase reporter assays and detected single nucleotide polymorphisms (SNPs) in the promoter region. Activity analysis discovered that the sequence from–187 to +174 is a basal promoter region, the sequence from–187 to–582 is a positive regulation region, whereas the sequence from–582 to–1321 is a negative regulation region. 11 SNPs were found in the promoter region of porcine GPIHBP1 , and the g.-796G>A SNP within the NF-l-binding site is associated with pig back fat thickness. The work performed here will serve as a valuable resource for discovering the expression regulation mechanism of GPIHBP1 and promoting further development of pig as a model organism for human cardiovascular disease research.
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Chen et al. (2018) studied this question.
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