What question did this study set out to answer?

To identify and characterize novel cell-surface receptors capable of binding high-density lipoprotein and mediating cellular lipid processing.

February 1, 2003Journal of Biological ChemistryOpen Access

Expression Cloning and Characterization of a Novel Glycosylphosphatidylinositol-anchored High Density Lipoprotein-binding Protein, GPI-HBP1

View Full Paper

Ask AI

Bookmark

Authors

RIRyoichi X. IokaJapan Science and Technology Agency MKMan‐Jong KangChonnam National University SKS. KamiyamaUniversity of Niigata Prefecture

Discussion

Member takes

Implication

In vitro study demonstrates high-affinity HDL binding by novel receptor GPI-HBP1 in cardiac and endothelial cells, indicating a tissue-specific pathway for selective lipid uptake.

Key Points

To identify and characterize novel cell-surface receptors capable of binding high-density lipoprotein and mediating cellular lipid processing.
Conducted expression cloning using fluorescently labeled high-density lipoprotein (HDL) to isolate cell-surface binding clones.
Characterized cloned cDNA sequences, sensitivity to phosphatidylinositol-specific phospholipase C, ligand specificity, and lipid transport versus cholesterol efflux.
Evaluated tissue and cell-type distribution across organs using Northern blotting and in situ hybridization.
Identified GPI-HBP1, a 228-amino-acid GPI-anchored protein with an Ly-6 motif that binds HDL with high affinity (calculated Kd = 2–3 µg/mL).
Demonstrated that GPI-HBP1 mediates selective lipid uptake without internalizing HDL apolipoproteins and lacks HDL-dependent cholesterol efflux capacity.
Detected highest transcript levels in cardiac muscle cells, with localized expression also observed in hepatic Kupffer cells, sinusoidal endothelium, bronchial epithelium, and alveolar macrophages.

Cite This Study

Ioka et al. (2003) studied this question.

synapsesocial.com/papers/6a82c376409efddc54d4ac8e https://doi.org/10.1074/jbc.m211932200

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates high-affinity HDL binding by novel receptor GPI-HBP1 in cardiac and endothelial cells, indicating a tissue-specific pathway for selective lipid uptake.

Expression Cloning and Characterization of a Novel Glycosylphosphatidylinositol-anchored High Density Lipoprotein-binding Protein, GPI-HBP1

Discussion

Member takes

Implication

Key Points

Cite This Study

Expression Cloning and Characterization of a Novel Glycosylphosphatidylinositol-anchored High Density Lipoprotein-binding Protein, GPI-HBP1

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider