March 1, 2000AJP Cell Physiology

Pharmacological activation of cloned intermediate- and small-conductance Ca2+-activated K+channels

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Why the study?

Does pharmacological activation with 1-EBIO, CZ, and ZOX increase the activity of cloned hIK1 and rSK2 channels in Xenopus oocytes?

Population

Xenopus oocytes heterologously expressing cloned intermediate-conductance and small-conductance…

Comparison

Pharmacological activation with… vs Absence of pharmacological activators

Design

Preclinical

Authors

CSColin A. SymeUniversity of Pittsburgh AGAaron C. GerlachDurham VA Medical Center ASAshvani K. SinghShanghai Institute for Science of Science

Discussion

Member takes

Implication

Activators of hIK1/rSK2 identified in oocytes; hypothesis-generating for vascular disease and cystic fibrosis therapies.

Structured PICO

Does pharmacological activation with 1-EBIO, CZ, and ZOX increase the activity of cloned hIK1 and rSK2 channels in Xenopus oocytes?

Population

Xenopus oocytes heterologously expressing cloned intermediate-conductance (hIK1) and small-conductance (rSK2) Ca2+-activated K+ channels

Intervention

Pharmacological activation with 1-ethyl-2-benzimidazolinone (1-EBIO), chlorzoxazone (CZ), or zoxazolamine (ZOX)

Comparator

Absence of pharmacological activators (baseline Ca2+-dependent activation)

Outcome

Channel activation characteristics (K(1/2), Hill coefficient, V(max), and event-duration analysis) measured using two-electrode voltage clamp and excised inside-out patch-clamp techniquessurrogate

1-EBIO, CZ, and ZOX are identified as the first known pharmacological activators of hIK1 and rSK2 channels, suggesting potential therapeutic utility for IK and SK channel openers in vascular diseases and cystic fibrosis.

Cite This Study

Syme et al. (2000) studied this question.

synapsesocial.com/papers/6a82c5b6abc87fc4bf1e1da7 https://doi.org/10.1152/ajpcell.2000.278.3.c570

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Pharmacological activation of cloned intermediate- and small-conductance Ca2+-activated K+channels

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Pharmacological activation of cloned intermediate- and small-conductance Ca2+-activated K+channels

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider