Human Circovirus in Patients with Hepatitis, Hong Kong

Figures

• 

  Figure 1. Results of screening in study of human circovirus in patients with hepatitis, Hong Kong. Ct, cycle threshold; HCirV, human circovirus.

• 

  Figure 2. Liver function test kinetics in 8 patients with hepatitis and human circovirus infection, Hong Kong. A) Patient 1; B) patient 2; C) patient 3; D) patient 4; E) patient 5; F) patient 6; G) patient 7; H) patient 8. Black line indicates ALT, and green line indicates bilirubin. Plus signs indicate days when human circovirus DNA was detected in blood, whereas dashes indicate days when human circovirus DNA was not detected in blood. ALT, alanine aminotransferase.

• 

  Figure 3. Hematoxylin and eosin stained liver sections of patients in study of human circovirus in patients with hepatitis, Hong Kong. A, B) Cholestasis with bile pigment in zone 3 canaliculi (arrows) in patient 5 at original magnification ×10 (A) and original magnification ×20 (B). C) Acidophil body (blue arrow) with neutrophils around ducts (green arrow) in patient 8; original magnification ×10.

• 

  Figure 4. Phylogenetic tree of representative circoviruses and human circoviruses from study of human circovirus in patients with hepatitis, Hong Kong. Maximum-likelihood phylogenetic tree was inferred from a multiple sequence alignment of complete representative Circovirus genomes and a mixture of full-length and partial genome sequences from this study (red text) using IQ-TREE (16). Three representative species of Cyclovirus were used as an outgroup for rooting the tree. Branch supports were assessed using the Shimodaira-Hasegawa–like approximate likelihood ratio test with 10,000 bootstrap replicates. Branches with <80% support were collapsed. Scale bar indicates nucleotide substitutions per site.

• 

  Figure 5. IgM and IgG immunoblots of 7 patients for whom sufficient serum was available from study of HCirV in patients with hepatitis, Hong Kong. P indicates positive control, mouse polyclonal serum raised against HCirV cap protein. N indicates HCirV PCR-negative human donor sample. HCirV, human circovirus.

• 

  Figure 6. Cell-binding assay showing binding of HCirV VLPs to PLC/PRF/5 cells in study of HCirV in patients with hepatitis. HEV VLPs (bHEV p239) is included as a positive control; SARS-CoV-2 NP protein is included as a negative control. Nuclei are counterstained with DAPI. Cap, capsid; HCirV, human circovirus; HEV, hepatitis E virus; NP, nucleoprotein; VLP, virus-like particle.