Rapid Affinity‐Based Fingerprinting of 14‐3‐3 Isoforms Using a Combinatorial Peptide Microarray

Specifying 14-3-3: A fragment-based combinatorial peptide microarray generates affinity-based fingerprints of seven mammalian 14-3-3 isoforms. High-affinity motifs are identified against the highly homologous isoforms. Putative 14-3-3σ-specific peptides were also delineated by a dual-color ratiometric screening strategy (see picture).