A method is described whereby fresh specimens of neural tissues are examined in squashes under the phase microscope. When squashed in contact with the sensitive tetrazolium derivative known as ‘INT’, granules of formazan are precipitated around the mitochondria within the neurocytoplasm. Application of these techniques to squashes of the spinal cord of the ray has given a direct demonstration of the dehydrogenase activity of mitochondria within fibres of the white matter of the cord.
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Arthur Hughes (1956) studied this question.
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