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A membrane colloid osmometer for measurement on samples down to 5 μl has been developed. Pellucid acryl plastic is used for most parts of the osmometer, allowing visual inspection of the reference fluid chamber. The osmometer base is attached directly to a Hewlett‐Packard pressure transducer, replacing the original dome of the transducer, thereby avoiding extra connections and leakage. The exposed membrane area has a diameter of 3 mm, and the membrane may be changed without completely disassembling the osmometer. With Amicon UM‐10 membranes and 0.9 % saline as reference fluid, the equilibration time for measurements on human sera was 2–4 min. Reduction of equilibration time to about 1 min was obtained by “priming” and flushing the membrane with serum ultrafiltrate. Reproducible measurements were obtained on samples down to 4–5 μl.
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Aukland et al. (1974) studied this question.
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