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Incubations of the 13‐ and 9‐hydroperoxides of linolenic acid with a flax acetone powder extract containing hydroperoxide isomerase resulted in the formation of 13‐hydroxy‐12‐oxo‐ cis ‐9,15‐octadecadienoic acid and 9‐hydroxy‐10‐oxo‐ cis ‐12,15‐octadecadienoic acid, respectively. The rate of formation of product from 13‐hydroperoxy linolenic acid was 36 times that from 9‐hydroperoxy linolenic acid. Analogous results were obtained with the 13‐ and 9‐hydroperoxides of linoleic acid. The results demonstrated the substrate specificity of flax hydroperoxide isomerase.
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Feng et al. (1979) studied this question.
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