Mannosyl residues from GDP-[14C]Man are transferred into liposomes and onto internal GDP if the liposomal membranes contain dolichyl phosphate and the enzyme GDP-Man: Dol-P mannosyl transferase. Experiments using a mixture of GDP-[14C]Man and [3H]GDP-Man excluded the possibility that the transmembrane translocation of [14C]mannose is due to a GDP-[14C]Manoutside ag GDPinside exchange reaction.
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Haselbeck et al. (1984) studied this question.
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