Why the study?
Ticagrelor is known to exert pleiotropic effects through unknown mechanisms, potentially influencing molecules involved in resolving inflammation.
Does ticagrelor influence CYP4F2 expression in HUVEC and HepG2 cells or exhibit antibacterial activity against E. coli?
Does ticagrelor influence CYP4F2 expression in HUVEC and HepG2 cells or exhibit antibacterial activity against E. coli?
Ticagrelor demonstrates pleiotropic effects by reducing CYP4F2 expression in endothelial and hepatic cell lines, but lacks antibacterial activity against resistant E. coli.
Answers are grounded in this paper and cite their sources.
Ticagrelor may modulate CYP4F2 in vitro; leaves open any clinical pleiotropy or antibacterial role pending in vivo data.
Background: Antiplatelet drugs, such as ticagrelor, which target platelet P2Y 12 receptors, are used for prevention of ischemic heart disease. Ticagrelor is also known to have pleiotropic effects of unknown mechanisms. Ticagrelor could influence the expression of molecules involved in resolution of inflammation. This study aimed to investigate if ticagrelor could change the expression of CYP4F2 and its encoded protein concentration and, additionally, to determine ticagrelor possible antibacterial activity against gram-negative bacteria. Methods: CYP4F2 expression was determined in HUVEC and HepG2 cell lines by qPCR. CYP4F2 protein concentration was determined by ELISA. Antibiotic susceptibility testing was performed using a disc diffusion method. Results: Ticagrelor was observed to reduce the expression of CYP4F2 in HUVEC and HepG2 cell lines. It also reduced CYP4F2 protein levels in HUVEC cells. Ticagrelor had no bactericidal activity against gram-negative third generation cephalosporin resistant E. coli . Conclusion: Ticagrelor reduced CYP4F2 protein concentration in HUVEC, and CYP4F2 expression in HUVEC and HepG2 cells, but had no effect on third-generation cephalosporin-resistant E. coli strains.
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Meškauskaitė et al. (2022) studied this question.
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