1H-NMR Studies of Metabolic Effects of Endo- and Exogeneous Compounds on Astroglia Cells Cultivated In Vitro

A 1 H-NMR method is described for the study of metabolic reactions in-vitro of neonatal cerebellar rat astroglia cells cultivated on micro carrier beads. In the NMR tube, the formation of metabolic effects on astroglia cells caused by addition of nutrients (pyruvate and fumarate) or neurotoxins can be monitored. Metabolic changes of both endogenous metabolites and toxins, 1,2-epoxybutane and ethyl acrylate are investigated. 1,2,4-Benzenetricarboxylate (BTC) served as an extracellular reference. Dy(ATP) -1 was used to depress signals from extracellular compounds. 6-Diazo-5-oxo-Lnorleucin was added to cause accumulation of conjugated intracellular metabolites to an extent that permitted detection by 1 H-NMR spectroscopy.