Determining the Reliability of Commoner Purification Methods and Mono-culturing Techniques for Microalgae

Background: The reliability of the purification process and monoculture technique for microalgae was investigated in this study. The study involves the isolation of microalgae from the environment of different localities to get the axenic culture of microalgae and test the reliability of other commonly used techniques. The vast usage of microalgae in industry and for the welfare of mankind demands the mono and pure culture of microalgae. Contaminants in the growth medium can alter the products which causes significant losses. Materials and Methods: Morphological study of any strain highly demands pure culture, so different techniques are being used, but only some techniques are reliable. Results: From the investigation, not a single technique is present which is entirely reliable, as contaminants are freely present in the universe so there are great chances of contamination. Conclusion: Comparatively single cell picking and dilution to extinction are effective methods for investigation purposes. These techniques are more reliable when combined with other techniques, for example, the addition of antibiotics or the use of ultraviolet radiation and washing methods compositely used with the above-mentioned techniques gave fruitful and reliable results.