An Amphotericin B–Fluorescein Conjugate as a Powerful Probe for Biochemical Studies of the Membrane

Not only skin deep: A fluorescein–amphotericin B conjugate with a new piperazine linker (see structure) was prepared and used as a probe of the biomembrane and the mechanism of action of amphotericin B both in vivo and in liposomal experiments. The amphotericin B analogue localizes at the membrane of yeast cells but is internalized by mammalian cells.