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HeLa S3 cells in suspension culture are resynchronized in each successive generation by exposure to 0.25m M thymidine. The resynchronization approach appears to be superior to single‐ and double‐block techniques because the period of effective inhibition of DNA synthesis is significantly shortened. Gram quantities of highly synchronous viable cells have been produced on a daily basis.
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Thilly et al. (1974) studied this question.
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