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The earlier experiments described by the writer upon the decomposition of glucose and mannitol by B. coli communis were open to the objection from the biological side that no attempt was made to distinguish those products which arose by enzyme action from those which might be more particularly associated in their formation with the growth and multiplication of the cells, and from the chemical side that certain substances of unknown composition, such as peptone, were employed, so that it was not possible to be sure that some of the products of fermentation had not been derived from this source. To overcome these objections the author has adopted a new plan of work which aims at separating the process of growth from that of fermentation.
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Egerton Charles Grey (1918) studied this question.
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