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The inorganic ions magnesium and potassium were required for optimal growth of Legionella pneumophila in a chemically defined medium composed of amino acids and inorganic salts. Optimum growth was obtained at concentrations of approximately 20 micrograms/mL (80 microM) MgSO4 X 7H2O and 150 micrograms/mL (2 mM) KCl. Comparable results were obtained with all six serogroups of L. pneumophila as well as with both laboratory-adapted and animal-passed strains.
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Tesh et al. (1982) studied this question.
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