Why the study?
Does Flotillin-1 deficiency affect CD8+ T cell morphology, migration, and clonal expansion in vivo?
Does Flotillin-1 deficiency affect CD8+ T cell morphology, migration, and clonal expansion in vivo?
Flotillin-1 plays a detectable but unexpectedly minor role in CD8+ T cell behavior and migration under physiological conditions in vivo.
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Flotillin-1 deficiency has limited impact on CD8+ T cell migration in mice; leaves open its relevance to human immunity or disease.
Flotillin-1 (Flot1) is a highly conserved, ubiquitously expressed lipid raft-associated scaffolding protein. Migration of Flot1-deficient neutrophils is impaired due to a decrease in myosin II-mediated contractility. Flot1 also accumulates in the uropod of polarized T cells, suggesting an analogous role in T cell migration. Here, we analyzed morphology and migration of naïve and memory WT and Flot1 -/- CD8 + T cells in lymphoid and non-lymphoid tissues with intravital two-photon microscopy, as well as their clonal expansion during antiviral immune responses. Flot1 -/- CD8 + T cells displayed minor alterations in cell shape and motility parameters in vivo but showed comparable homing to lymphoid organs and infiltration into non-lymphoid tissues. Taken together, Flot1 plays a detectable but unexpectedly minor role for CD8 + T cell behavior under physiological conditions.
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Ficht et al. (2018) studied this question.
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