PPARα and PPARγ Regulation of Liver and Adipose Proteins in Obese and Dyslipidemic Rodents

Zucker fatty rats and ob / ob mice are both frequently used hyperlipidemic and insulin-resistant spontaneous genetic models of obesity. We used them to study the effect of PPAR agonists on the protein-expression level in liver and white adipose tissue. PPARα-agonist treatments of the rats resulted in that 27% of the quantified hepatic proteins were altered; implicating pronounced peroxisome proliferation and increase in capacity for β-oxidation of fatty acids although no correction of plasma triglycerides were obtained. On treatment with PPARγ agonists, adipose proteins were regulated to a much larger extent in the rats compared to mice, 18% and 2%, respectively.