Gibberellin A1 (GA1), which was identified as the major GA from the GA-producing fungus Phaeosphaeria sp. L487, was accumulated in the culture with a maltose-yeast extract medium, its amount in the culture filtrate being about 50 mg per liter after a 3-week culture. The new fungal biosynthetic pathway to GA1 from GA9 via GA4 was elucidated by feeding experiments with synthetic [17-2H2]GA9 and [17-2H2]GA4.
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Kawaide et al. (1993) studied this question.