Covalent linkage of the three calix[4]arene units in hydrogen-bonded assemblies 13•(DEB)6via a threefold ring closing metathesis (RCM) reaction quantitatively converts the dynamic assemblies into covalent systems (123-membered macrocycles) that can be easily characterized using MALDI-TOF MS and HPLC.
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Cardullo et al. (2000) studied this question.
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