High-Resolution Microradiography Using Low-Voltage X-Rays

Mathematical considerations of the concept of extremely high resolution in microradiography have allowed the derivation of optimal conditions for studies of biological material. Specimen thickness must be kept below 4 microns, and as a consequence very low voltages over the x-ray tube are needed. Using a special method for preparing the specimen for analysis, it has been possible to obtain microradiograms at x-ray potentials of a few hundred volts. The absolute resolution of the x-ray image is comparable to that of the light microscope.