¹³C NMR spectroscopy of keratan sulphates: Assignments for the reduced form of a repeat unit tetrasaccharide derived from keratan sulphate by keratanase digestion and partial assignments for two fucosylated pentasaccharides

A full assignment of the 60°C 13 C NMR spectrum for the reduced form of a repeat unit β‐linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6SO 4 )β 1–3 Gal(6SO 4 )β 1–4 GlcNAc(6SO 4 )β 1–3 ‐Gal‐ol, was achieved via two‐dimensional C/H correlation and 1 H COSY experiments, and a complete first‐order analysis of the 600 MHz proton spectrum. Partial 13 C assignments for two fucosylated keratan sulphate pentasc‐charides derived using hydrazinolysis and nitrous acid treatment are discussed.