Summary The p‐anisidine method for the determination of gossypol in crude cottonseed oils has been reinvestigated and modified to make it applicable to all crude oils obtained by the newer methods of processing cottonseed. The modifications included a change in the composition of the solvent, a higher reaction temperature, and the use of a more concentrated panisidine reagent. The modified method was found satisfactory where different colorimeters and spectrophotometers were used for measuring the color developed. Comparison of aniline and p‐anisidine as reagents for the analysis of gossypol pigments showed that the presence of modified gossypol in some crude oils resulted in an over‐correction for background absorption and led to significant errors when aniline was used as the color development agent.
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Pons et al. (1956) studied this question.
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