The fluorescent probe ROFRET contains a Bodipy molecular rotor connected through a short triazole-based spacer to a fully alkylated Bodipy. Förster resonance energy transfer takes place from the rotor to the other Bodipy, and is enhanced to a limiting value as the viscosity of the solvent increases. Time-resolved spectroscopy and steady-state studies are consistent with both forward and reverse energy transfer, and delayed fluorescence.
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Bai et al. (2014) studied this question.
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