Lactobacillus mulieris sp. nov., a new species of Lactobacillus delbrueckii group

One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161M T , was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161M T belonged to the genus Lactobacillus . Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161M T and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii , respectively. The major fatty acids of strain c10Ua161M T were C 18 : 1 ω 9c (65.0%), C 16 : 0 (17.8%), and summed feature 8 (10.2 %; comprising C 18 : 1 ω7 c , and/or C 18 : 1 ω6 c ). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161M T represents a novel species of the genus Lactobacillus , for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161M T (=CECT 9755 T =DSM 108704 T ).