Assisted oocyte activation does not overcome recurrent embryo developmental problems

A Cardona Barberán; Davina Bonte; Annekatrien Boel; Vanessa Thys; R Paredis; F Machtelinckx; Petra De Sutter; Ilse De Croo; Luc Leybaert; Dominic Stoop; Paul Coucke; Frauke Vanden Meerschaut; Björn Heindryckx

doi:10.1093/humrep/dead051

March 17, 2023Human Reproduction

Assisted oocyte activation does not overcome recurrent embryo developmental problems

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Key Points

To assess whether assisted oocyte activation (AOA) using calcium chloride and ionomycin improves blastocyst development in couples experiencing recurrent embryonic developmental arrest despite normal fertilization after ICSI.
Interventional prospective trial (NCT03354013) comparing 17 ICSI-AOA cycles to 44 prior standard ICSI cycles in 42 couples with normal fertilization (≥60%) but poor blastocyst formation (≤15%) in at least two prior cycles.
Intervention comprised CaCl2 injection alongside double ionomycin exposure, supplemented by mouse oocyte calcium release analysis of patient sperm.
Couples underwent targeted genetic screening for maternal subcortical complex genes (TLE6, PADI6, NLRP2, NLRP5, NLRP7, KHDC3L) and paternal PLCZ1.
ICSI-AOA achieved a blastocyst development rate of 13.74% (18/131) compared to 9.35% (30/321) in prior ICSI cycles (P = 0.18).
Fertilization rates were 68.95% (131/190) with AOA versus 76.25% (321/421) in prior cycles (P = 0.06); live birth rates were 23.53% (4/17) versus 15.91% (7/44) (P = 0.48).
Genetic screening identified 10 unique heterozygous variants of uncertain significance across maternal genes in 14 women, plus a homozygous PLCZ1 mutation (p.Ser500Leu) in one male patient.

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Implication

Prospective clinical trial finds assisted oocyte activation does not improve blastocyst formation in recurrent developmental failure, questioning broad usage.

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Cite This Study

Barberán et al. (2023) studied this question.

synapsesocial.com/papers/6a83c201d4bda846d66a90ed https://doi.org/10.1093/humrep/dead051

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Assisted oocyte activation does not overcome recurrent embryo developmental problems

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Assisted oocyte activation does not overcome recurrent embryo developmental problems

Key Points

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Implication

Discussion

Cite This Study

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