Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.