New Robust Redox and Conducting Polymer Modified Electrodes for Ascorbate Sensing and Glucose Biosensing

A novel modified electrode with a conducting film containing poly(3,4‐ethylenedioxythiophene) (PEDOT) plus poly(methylene blue) (PMB) on a glassy carbon electrode (GCE) (PEDOT/PMB/GCE) has been developed, and application illustrated as electrochemical sensor for ascorbate and biosensor for glucose. Electrocatalytic oxidation at 0.0 V vs. SCE was successfully used for the determination of ascorbate in real samples. Glucose biosensors containing glucose oxidase (GOx) immobilized on PMB/PEDOT electrodes exhibit enhanced sensitivity relative to PEDOT ones. The new robust biosensor architecture shows a far superior operational and storage stability relative to PMB alone, enabling excellent preservation of enzyme activity during more than one month.