Targeting proteins: Metal phosphate nanoparticles (see scheme; NHS-biotin=biotinamidohexanoyl-6-amino-hexanoic acid N-hydroxy-succinimide ester) based on an apoferritin template have been synthesized and used as biological labels for electrochemical immunoassay. The template offers a simple and convenient route for the preparation of metallic nanoparticle labels under mild conditions. The assay method is ultrasensitive (detection limit as low as 77 fm); simultaneous detection of multiple protein targets can be performed by using different labels.
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Liu et al. (2006) studied this question.
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