Diverse characters of Brennan’s paw incision model regarding certain parameters in the rat

Figures

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  Fig. 1. Activated protein C (10 μg) was administered by intra-wound route using a sterile micropipette, only once in each rat during the experiment.

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  Fig. 2. Estimation of peroxidase component of cyclooxygenase (COX) enzymes by calorimetric method. Control animals (n = 4) were sacrificed, before (basal) and at specific time points after incision (between 2 hr and day 3) for estimating COX activity. Maximum increase was noted at 8 hours, when COX-2 activity was greater than COX-1. Pooled tissues (n = 4) was evaluated at each time point and thus statistical analysis could not be performed.

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  Fig. 3. Biochemical estimation of leukotriene B4 (LTB4) by competitive ELISA in control group of animals before (basal) and after incision (between 2 hr and day 3). Maximum increase was observed at 8 hours compared to basal level. The increase persisted up to day 3. Values (n = 4) are represented as mean ± standard error of mean. *P < 0.05, **P < 0.01, ***P < 0.001.

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  Fig. 4. Estimation of hemodynamic parameters in control group of animals, before (baseline) and after incision (between 2 hr and day 5). Blood pressure (A) and heart rate (B) were not affected compared to baseline data. Values are expressed as mean ± standard error of mean. n = 6 at each time point.

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  Fig. 5. Assessment of metabolic function in control animals. Time of incision is indicated (line arrow). Feeding behaviour was affected at days 1 and 3 (A) and drinking on day 1 (B) after incision. All values were compared to baseline intake. Values are represented as mean ± standard error of mean. Food consumption was expressed as g/day. n = 6 at each time point. *P < 0.05, **P < 0.01, ***P < 0.001.

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  Fig. 6. Intra-wound administration of protein C (10 μg) only once, resulted in a decrease in guarding score (A) but did not affect mechanical allodynia (B) or thermal hyperalgesia (C). Treatment with activated protein C reduced guarding score from 2 hours to day 2 in comparison to control group. Values (n = 6) are represented as mean ± standard error of mean. Day 1 to 7 refers to days 1 to 7 (***P < 0.001).