Various purification procedures for folic acid were investigated. The criteria of purity were a negative Bratton-Marshall test and the absence of fluorescent spots on paper chromatograms. Since no method provided a pure product, a procedure consisting of cellulose column chromatography followed by filtration through charcoal was developed.
No takes yet. Share an insight, caveat, or question.
Sakami et al. (1959) studied this question.