Laboratory Evaluation for Urine Identification Card in the Automated Microbial System

This is the authors' abstract. We don't add key points for this paper.

Automation of microbiology tests is a matter of great importance in the field of clinical laboratory work. Various tests were performed to evaluate the auto microbic system (AMS, Vitek System, Inc), particularly the specificity and sensitivity of the urine identification card. With the urine identification card, it is possible to identify 9 species of bacteria which can be isolated at high frequency and enumerate the number of colony forming units in the urine sample. Used as test materials were seed specimens prepared from standard strains and 807 clinical urine samples. Evaluation was made by comparison of results obtained by the clinical microbiology laboratory (CML) method and AMS.The results showed agreement in more than 95% for the various species of bacteria with a small number presenting false positive finding. In order to make identification with AMS, 104-5/ml of colony forming units (CFU) for each species, and detection cannot be made with less than 103/ml of CFU in unine.The card is inoculated with the sample directly, and as the respective microwells have high selectivity, Isolated culture is not necessary. The results can be get within 13 hours, which is much more rapid than conventional method, and thus it is considered to be quite promicing as a clinical procudure.