Abstract convenient method based on the utilization of isopentenyl pyrophosphate (IPP) isomerase was developed to facilitate the discrimination of the prochirality of the hydrogen eliminated from IPP during prenyltransferase reaction. E,E-Farnesyl pyrophosphate synthetase of pumpkin was shown to catalyze the addition of allylic moiety to the si face of the double bond of IPP with loss of the 2-pro-R hydrogen.
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Ito et al. (1986) studied this question.